How To Make Stevioside Powder？

Understanding the Ingredients

 

Stevioside powder is made from the leaves of the stevia rebaudiana plant. Although it belongs to the same family as chrysanthemum, it is rich in a class of diterpenoid compounds called steviol glycosides, of which RA (Reb-A) and STV contribute significantly to its sweetness. The tenderer the leaves and the more sunlight they receive, the higher their glycoside content. Stevia seedlings purchased online are easily viable, and potted plants on balconies can yield two seasons of leaves.

 

How to make stevioside powder?

 

The following recipe uses 50 grams of dried leaves as an example, and the entire process takes approximately 3 hours.

 

Low-Temperature Ultrasonic Extraction

After removing the stems and crushing the dried leaves, soak them in 500 mL of 60°C purified water. Add 1 gram of vitamin C (for antioxidant and color protection). Place the leaves in an ultrasonic cleaner (a home jewelry cleaner is suitable) and shake for 20 minutes at 120W. Ultrasonic cavitation breaks down the waxy layer on the leaf surface, allowing the glycosides to dissolve rapidly. Filter the liquid, return the residue to the pot, and combine the two filtrates to approximately 800 mL.

 

Flocculation and Impurity Removal

Heat the filtrate to 80°C and add 0.5 grams of food-grade chitosan (available online for about 2 cents/gram). Stir slowly for 5 minutes, then let it sit for 30 minutes. Chitosan is positively charged and can complex with colloids such as polyphenols and chlorophyll, forming a flocculent precipitate. If chitosan is unavailable, one egg white can be used as a substitute, though with slightly less effectiveness. Then, double filter with gauze and coffee filter paper to obtain approximately 750 mL of clear, light yellow liquid.

 

Decolorization and Debittering

Industry uses macroporous resin adsorption, while households can use the "activated carbon + alcohol two-step method":

① Add 3 grams of powdered activated carbon to the concentrated syrup, stir at 60°C for 10 minutes, and filter. The color will change from brownish yellow to light yellow.

② Add 50 mL of 95% ethanol. Steviosides are readily soluble in alcohol, while bitter substances are less soluble. Let stand at room temperature for 20 minutes, then filter and recover the alcohol (which can be distilled and reused), resulting in a nearly colorless, transparent syrup.

 

Drying and Powdering

Pour the refined syrup into a silicone mold and spread it into 2 mm thin slices. Place in a household freeze dryer (pre-freeze at -40°C, then apply vacuum for 12 hours) or dry in a hot air oven at 60°C for 4 hours. After embrittlement, crush the mixture, pulverize with a wall crusher, and pass through an 80-mesh sieve. The final product is approximately 4.5 grams of snow-white stevia powder, with a yield of 9% and a purity of ≥82% as measured by a simple refractometer, fully meeting export-grade requirements.

 

stevioside powder manufacturer

 

Sost biotech has become the first choice for stevia powder buyers due to its continuous passing of US FDA and EU organic certification.we supply:

 

RA60	Total glycosides ≥60%,Reb-A≥60%
RA97	Reb-A≥97%
Organic RA97	Organic Reb-A≥97%

 

Contact Email:info@sostherbusa.com